ABSTRACT
An investigation was done to determine the prevalence of known intestinal pathogens in the stools and duodenal juice of diarrheal children as well as a study of their pathogenicity and mechanism of action. Both duodenal juice and stool samples were collected from 150 cases and stools alone were obtained from a further number of 75 cases. All samples were examined for the presence of enteropathogenic bacteria as well as rotavirus antigen. Duodenal juice samples were also subjected to a viable count of its aerobic bacterial content. Known pathogens were detected in the stools of 43.5% of all 225 cases and in 38% of the duodenal juice samples of 150 cases. These included ETEC, EPEC, Campylobacter, Salmonella, Shigella and Rotavirus from stools and ETEC, EPEC, Salmonella, Rotavirus, Klebsiella pneumoniae, Pseudomonas and Citrobacter from juice. ETEC was the most frequently isolated pathogen from both specimens [8.9% from stools and 12% from duodenal juice] followed by EPEC [5.8% and 6.7%, respectively]. While, Vibrio, EIEC and Y. enterocolitica were not isolated from any of the specimens in all cases
Subject(s)
Duodenum/microbiology , Mass ScreeningABSTRACT
Eleven out of 13 strains of enterotoxigenic E. coli resistant to antibiotics were tested for their ability to transfer both antibiotic resistance and interotoxigenicity to E. coli C600 by conjugation experiments. Eight strains [73%] were able to transfer resistance to various antibiotics and 5 of these [62.5%] were also able to cotransfer enterotoxigenicity to the standard recipient. All strains were also tested for colicin production, but none proved colicinogenic and four strains were found to possess CFA/I
Subject(s)
Drug Resistance, Microbial , EnterotoxinsABSTRACT
A total of 475 salmonella strains was studied for colicin production. Forty-three colicin producers were detected. These comprized 13 strains of S. typhi, 23 of S. wien, 4 of S. montevideo, one of each of S. typhimurium, S. johannesburg and S. ohio. Eighteen strains of S. wien produced colicins related to type Ib and the colicinogenic S. typhi and S. typhimurium produced colicins related to type I. Two strains of S. montevideo produced colicins related to colicin I and two strains produced colicins related to colicin E